polyclonal antibodies against p53 Search Results


polyclonal antibodies against phosphorylated p53 ser-6  (AnaSpec)
90
AnaSpec polyclonal antibodies against phosphorylated p53 ser-6
Polyclonal Antibodies Against Phosphorylated P53 Ser 6, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibodies against phosphorylated p53 ser-6/product/AnaSpec
Average 90 stars, based on 1 article reviews
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rabbit polyclonal antibody against p53  (Abiocode Inc)
90
Abiocode Inc rabbit polyclonal antibody against p53
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Rabbit Polyclonal Antibody Against P53, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibody against p53/product/Abiocode Inc
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibody against p53 - by Bioz Stars, 2026-03
90/100 stars
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polyclonal antibodies against p53 peptides phosphorylated residues  (AnaSpec)
90
AnaSpec polyclonal antibodies against p53 peptides phosphorylated residues
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Polyclonal Antibodies Against P53 Peptides Phosphorylated Residues, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibodies against p53 peptides phosphorylated residues/product/AnaSpec
Average 90 stars, based on 1 article reviews
polyclonal antibodies against p53 peptides phosphorylated residues - by Bioz Stars, 2026-03
90/100 stars
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polyclonal antibodies against p53 peptides with phosphorylated residues serine 6  (AnaSpec)
90
AnaSpec polyclonal antibodies against p53 peptides with phosphorylated residues serine 6
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Polyclonal Antibodies Against P53 Peptides With Phosphorylated Residues Serine 6, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibodies against p53 peptides with phosphorylated residues serine 6/product/AnaSpec
Average 90 stars, based on 1 article reviews
polyclonal antibodies against p53 peptides with phosphorylated residues serine 6 - by Bioz Stars, 2026-03
90/100 stars
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goat polyclonal antibodies against p53  (Boehringer Mannheim)
90
Boehringer Mannheim goat polyclonal antibodies against p53
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Goat Polyclonal Antibodies Against P53, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat polyclonal antibodies against p53/product/Boehringer Mannheim
Average 90 stars, based on 1 article reviews
goat polyclonal antibodies against p53 - by Bioz Stars, 2026-03
90/100 stars
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polyclonal antibodies against phosphorylated p53  (AnaSpec)
90
AnaSpec polyclonal antibodies against phosphorylated p53
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Polyclonal Antibodies Against Phosphorylated P53, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibodies against phosphorylated p53/product/AnaSpec
Average 90 stars, based on 1 article reviews
polyclonal antibodies against phosphorylated p53 - by Bioz Stars, 2026-03
90/100 stars
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polyclonal monoclonal antibody against p53  (Becton Dickinson)
90
Becton Dickinson polyclonal monoclonal antibody against p53
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Polyclonal Monoclonal Antibody Against P53, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal monoclonal antibody against p53/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
polyclonal monoclonal antibody against p53 - by Bioz Stars, 2026-03
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polyclonal antibody zfp53-n against the 1e112 amino acid region of p53  (Abmart Inc)
90
Abmart Inc polyclonal antibody zfp53-n against the 1e112 amino acid region of p53
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Polyclonal Antibody Zfp53 N Against The 1e112 Amino Acid Region Of P53, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal antibody zfp53-n against the 1e112 amino acid region of p53/product/Abmart Inc
Average 90 stars, based on 1 article reviews
polyclonal antibody zfp53-n against the 1e112 amino acid region of p53 - by Bioz Stars, 2026-03
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primary rabbit polyclonal antibody against human p53 that cross-reacts with feline p53  (Novocastra)
90
Novocastra primary rabbit polyclonal antibody against human p53 that cross-reacts with feline p53
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Primary Rabbit Polyclonal Antibody Against Human P53 That Cross Reacts With Feline P53, supplied by Novocastra, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary rabbit polyclonal antibody against human p53 that cross-reacts with feline p53/product/Novocastra
Average 90 stars, based on 1 article reviews
primary rabbit polyclonal antibody against human p53 that cross-reacts with feline p53 - by Bioz Stars, 2026-03
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rabbit polyclonal antibodies against p53 201  (Wanleibio)
90
Wanleibio rabbit polyclonal antibodies against p53 201
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
Rabbit Polyclonal Antibodies Against P53 201, supplied by Wanleibio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal antibodies against p53 201 - by Bioz Stars, 2026-03
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p53 (ab-7) sheep polyclonal antibody against p53  (Amersham Life Sciences Inc)
90
Amersham Life Sciences Inc p53 (ab-7) sheep polyclonal antibody against p53
Establishment of <t>p53</t> stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of <t>p53</t> <t>protein</t> level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.
P53 (Ab 7) Sheep Polyclonal Antibody Against P53, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p53 (ab-7) sheep polyclonal antibody against p53/product/Amersham Life Sciences Inc
Average 90 stars, based on 1 article reviews
p53 (ab-7) sheep polyclonal antibody against p53 - by Bioz Stars, 2026-03
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Image Search Results


Establishment of p53 stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of p53 protein level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem cell-mediated suppression of hypertrophic scarring is p53 dependent in a rabbit ear model

doi: 10.1186/scrt526

Figure Lengend Snippet: Establishment of p53 stable knockdown in rabbit mesenchymal stem cells. Passage 4 of mesenchymal stem cells (MSCs) generated from femur bone marrow was transduced with lentiviral particles containing p53 shRNA or control (Ctrl) shRNA. (A) Stable clones were harvested after puromycin selection, and cells of passages 3 to 9 were used for the following tests. Scale bar, 500 μm. (B) Representative western blot image of p53 in MSCs. (C) Quantification of p53 protein level in western blot by densitometry. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as internal control ( n = 3, * P <0.01 vs. control group). (D) 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay showed no significant difference in cell viability between the two groups. P >0.05. (E) Representative images of cell colony formation. Viable cells (2,000 cells/well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Scale bar, 1 cm. (F) Quantification of the number of colony formation in p53 shRNA and control shRNA groups. P >0.05. (G) Flow cytometry analysis of cell surface markers. Blue line, negative control. (H) Differentiation capacity of the cells. Both control shRNA-transduced and p53 shRNA-transduced MSCs differentiated into mineralizing cells stained with alizarin red, or adipocytes stained with oil red. Scale bar, 200 μm. Data presented as mean ± standard error of the mean, n = 3 individual experiments.

Article Snippet: Rabbit p53 shRNA lentiviral particles, control shRNA lentiviral particles and rabbit polyclonal antibody against p53 were purchased from Abiocode Bio-Technology (Shanghai, China).

Techniques: Generated, Transduction, shRNA, Clone Assay, Selection, Western Blot, MTS Assay, Staining, Flow Cytometry, Negative Control